HANDAYANI, KRISTINA (2020) UJI AKTIVITAS ANTIBAKTERI FRAKSI BATANG PEPAYA (Carica Papaya Linn.) TERHADAP BAKTERI Staphylococcus Aureus ATCC 25923. Undergraduate thesis, Stikes Karya Putra Bangsa Tulungagung.
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Skripsi- Kristina Handayani.pdf Download (1MB) |
Abstract
Infectious disease is an important disease, especially in developing countries like Indonesia. Infectious diseases can be caused by pathogenic microorganisms such as the bacterium Staphylococcus aureus ATCC 25923. Skin infections due to S.aureus ATCC 25923 has been found 179 cases in Jakarta. Infectious diseases can be treated with antibiotics, but antibiotics now experience resistance due to the inappropriate use of antibiotics. Therefore, other alternatives such as are needed papaya stem which can be used as an antibacterial against S.aureus ATCC 25923. The purpose of this study was to determine the antibacterial activity against S.aureus ATCC 25923, knowing the most active fraction of papaya stem, knowing the content of compounds in the most active fraction of papaya stem and knowing the MIC value in the most active fraction of papaya stem fraction. The research method used is experimental. Papaya stem sample extracted using a method soxhletation with 96% ethanol followed by fractionation using three solvents namely aquadestilate, dichloromethane, and n-hexane. The positive control used is clindamycin and the negative control is the solvent appropriate. The most active fraction is tested to determine the Minimum Inhibition Concentration (MIC) using a visual and observation Uv-Vis spectrophotometer. Statistical analysis is done with Kruskal Wallis and Mann Whitney. The results of testing the antibacterial activity of papaya stem fractions showed the presence of antibacterial activity against S.aureus ATCC 25923. The inhibitory zone results in aquadestilate, dichloromethane, and n-hexane fractions were 20.63 ± 0.28 mm, 17.86 ± 0.18 mm, and 15.33 ± 0.47 mm, respectively. The papaya stem fraction which has the most active inhibition zone is the aquadestillate fraction. The antibacterial activity is thought to originate from a flavonoid, tannin, and saponin compounds activity in papaya stem fraction. The Minimum Inhibitory Concentration value of the aquadestillate fraction is 0.312%. Keywords: Antibacterial, Carica papaya L., Minimum Inhibitory Concentration, Staphylococcus aureus ATCC 25923
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Uncontrolled Keywords: | Antibakteri, Carica papaya L., Konsentrasi Hambat Minimum, Staphylococcus aureus ATCC 25923 |
| Subjects: | Q Science > Q Science (General) Q Science > QK Botany R Medicine > R Medicine (General) |
| Divisions: | Program Studi > S1 Farmasi |
| Depositing User: | Surya Hadi Prasetyo |
| Date Deposited: | 24 May 2022 03:38 |
| Last Modified: | 24 May 2022 03:38 |
| URI: | http://repository.stikes-kartrasa.ac.id/id/eprint/73 |
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