HIDAYAH, NURUL (2020) UJI AKTIVITAS ANTIBAKTERI FRAKSI DAUN BIDURI (Calotropis gigantea L.) TERHADAP Staphylococcus aureus. Undergraduate thesis, Stikes Karya Putra Bangsa Tulungagung.
![[img]](http://repository.stikes-kartrasa.ac.id/style/images/fileicons/text.png) |
Text
SKRIPSI NURUL HIDAYAHH.pdf Download (919kB) |
Abstract
Staphylococcus aureus is a Gram positive, clustered form. Staphylococcus aureus is a major cause of nosocomial infections, food pisoning, and toxic shock syndrome. Staphylococcus aureus infection cant occur when the immune system is weakened. Antibiotics used as one of the ways to treat infection, but inappropriate use of antibiotics will cause resistance. Traditional medicine made from plant extract can be used as an alternative way to treat the infection. Traditional medicines derived from plants have far lower side effects than chemical drugs. One of the plants that can be used as medicinal ingredients is biduri plant (Calotropis gigantea L.). The purpose of this study was to determine the activity of Aquadestilata, ethyl acetate, and n-hexsane fractions of biduri leaf extract as an antibacterial against Staphylococcus aureus bacteria. Calotropis gigantea leaves were extracted using maceration methods. Alcohol 70% was used as a solvent in the maceration procedure. Extract that we obtained, then undergone in fractionation procedure using Aquadestilata, ethyl acetate, and n-hexane as a solvent. The antibacterial activity of Calotropis gigantea leaves fraction was tested using the disc diffusion method with a series concentration of 10%, 20%, and 30%. One way ANOVA was used to statistically analyze the data obtained. In statistical analysis, we could found out whether the variation of the various concentration of Calotropis gigantea leaves fraction has any positive effect or not, againts the growth of Staphylococcus aureus bacteria. The results of the research that has been done shown that Aquadestilata and ethyl acetate fraction have antibacterial activity. Aquadestilata fraction was the most active fraction that inhibits the activity of the bacteria Staphylococcus aureus. That was because it contains flavonoid compounds, tannins, and saponins. The optimum concentration of Calotropis gigantea leaves fractions to inhibits the growth of bacteria Staphylococcus aureus was 10%, with an average inhibition zone was 6,33mm. Keywords: antibacterial, Bithorn leaves, diffusion, fractionation, Staphylococcus aureus.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Uncontrolled Keywords: | antibakteri, daun biduri, difusi, fraksinasi, Staphylococcus aureus. |
| Subjects: | Q Science > Q Science (General) Q Science > QK Botany R Medicine > R Medicine (General) |
| Divisions: | Program Studi > S1 Farmasi |
| Depositing User: | Surya Hadi Prasetyo |
| Date Deposited: | 25 May 2022 04:13 |
| Last Modified: | 25 May 2022 04:13 |
| URI: | http://repository.stikes-kartrasa.ac.id/id/eprint/80 |
Actions (login required)
 |
View Item |